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以下文字为某篇科研论文摘要,请分析此摘要的结构组成如背景、目的、方法、结果、结论,需指出每部分结构所包含的文字起始,并根据摘要内容为该论文拟定题目。For decades, the risks of exposure to infectious diseases in recreational beaches has been addressed by measuring abundance of fecal indicator bacteria from water samples through culture methods. Recently, analyses of sand samples for beach quality assessments have been developed as a complement for monitoring recreational waters. In parallel, the growing use of molecular techniques for environmental monitoring has led to obtaining rapid results on the detection of pathogenic genes, providing more accurate information about the health risk of exposure to contaminated sand. The aim of this work was to determine the relationship between the fecal indicator abundance in water and sand. The presence of Shiga toxin-producing Escherichia coli (STEC) by analyzing samples from touristic beaches by culture-dependent (fecal coliforms assay) and culture-independent (real-time PCR of stx1, stx2 and eae genes) methods. We found high CFUs in water and sand along the studied beaches, without an association with sanitation network, nor the level of urbanization. Nevertheless, the highest values (5300 CFU/100mL) was detected in city A. The presence of STEC virulence genes was confirmed in 8 out of 20 sand samples, associated with the lack of sanitation network. The recreational use of sandy beaches may imply a risk to the health of its users, especially near streams and creek outflows, demonstrating the importance of monitoring sand bacteriological quality, and the usefulness of directly monitoring pathogens by means of molecular tools.

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摘要结构分析

背景:摘要的背景部分从开头到“providing more accurate information about the health risk of exposure to contaminated sand.” 这部分介绍了休闲海滩暴露于传染病的风险,传统上通过培养方法测量水中粪便指示菌的丰度,以及沙子样本分析作为监测娱乐水域的补充,以及分子技术在环境监测中的日益增长的使用。

目的:目的部分从“The aim of this work was to determine”开始,到“in water and sand”结束。这部分明确了研究的目的是确定水中粪便指示菌丰度与沙子中丰度之间的关系。

方法:方法部分从“The presence of Shiga toxin-producing Escherichia coli (STEC) by analyzing samples”开始,到“culture-independent (real-time PCR of stx1, stx2 and eae genes) methods”结束。这部分描述了研究中使用的方法,包括依赖培养的方法(粪便大肠杆菌群测定)和不依赖培养的方法(实时PCR检测stx1、stx2和eae基因)。

结果:结果部分从“We found high CFUs in water and sand along the studied beaches”开始,到“The presence of STEC virulence genes was confirmed in 8 out of 20 sand samples”结束。这部分报告了研究发现的高CFU值,以及在20个沙子样本中有8个确认存在STEC毒力基因。

结论:结论部分从“The recreational use of sandy beaches may imply a risk”开始,到摘要的结尾。这部分得出结论,沙子细菌质量的监测和使用分子工具直接监测病原体的重要性。

论文题目拟定

根据摘要内容,可以为该论文拟定以下题目:

“沙子和水中粪便指示菌丰度的相关性研究:以旅游海滩为案例”

这个题目概括了研究的主要内容和目的,即研究沙子和水中粪便指示菌的丰度之间的关系,并以旅游海滩作为研究案例。同时,它也暗示了研究的潜在应用和重要性,即沙子质量的监测和分子工具的使用对于保护海滩使用者的健康至关重要。39

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